Source: UNIVERSITY OF FLORIDA submitted to NRP
GENETIC IMPROVEMENT OF HORTICULTURAL CROPS FOR IMPROVED SENSORY CHARACTERISTICS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1003388
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 2, 2014
Project End Date
Jun 22, 2019
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF FLORIDA
G022 MCCARTY HALL
GAINESVILLE,FL 32611
Performing Department
Environmental Horticulture
Non Technical Summary
In the US, we are faced with an obese population that spends less time outdoors and more time indoors. As a way to bring a healthier andbetter quality of life to our citizens, we believe that foundational experiments needs to be conducted to understand how to help people enjoy the plants they eat and the plants they surround themselves with more than they do now. Instead of delivering novel new plants, fruits and vegetables based on the decisions of the plant geneticist, we are interested in finding our which characteristics of plants appeal to and detract from the human experimence the most. By understanding these characteristics, we belive we can select new varieties that people will like more - as a result they will eat healthier and surround themselves with plants that make them feel better, hence improved health and quality of life.
Animal Health Component
30%
Research Effort Categories
Basic
30%
Applied
30%
Developmental
40%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20621231081100%
Knowledge Area
206 - Basic Plant Biology;

Subject Of Investigation
2123 - Bedding/garden plants;

Field Of Science
1081 - Breeding;
Goals / Objectives
The main goal of this project is to conduct research focused on bridging the gap between basic and applied science through the genetic improvement of various horticultural crops. Using a concept known as Consumer-assisted Selection, we conductconsumer analysis studiesto determine which sensory characters of different crops have the most or least appeal. By comparing this information with analytical chemistry data for fragrance, flavor and texture, we can find out which compounds are responsible for the most or least desirable characters. This information is then used to help make genetic selections for new varieties with maximum sensory appeal. We also conduct experiments focused on the applied side of horticultural crop production with an emphasis on determining the best ways to get crops through the supply chain with maximum sensory appeal.
Project Methods
We are currently working on the genetic improvement of various edivble and ornamental horticulture crops. We start with a psychophysics approach, which allows us to understand what sensory aspect of a plant humans like or dislike the most. Next, we conduct analytical chemistry experiments to determine the chemical analysis of a given fruit, flower or vegetable. Next we conduct multivariate statistical analysis to determine which chemicals are most responsible for consumer liking of the crop. We then use that information to help us select the plants with maximum consumer appeal. We also conduct experiments focused on determining the effects of production or storage environment of quality of horticultural crops. This allways us to not only better control the genetics, but also control the environment as a means to deliver the highest quality horticultural crops.

Progress 10/01/17 to 09/30/18

Outputs
Target Audience:-Human sensory scientists -Plant breeders and biotechnologists -Plant producers -Plant shippers and handlers -Plant marketers -Food scientists -Consumers of plant-based food products ? Changes/Problems:The only major changes we have made recently is to do more conventional plant breeding instead of doing more basic laboratory research focused on molecular biology.This shift of efforts is not a shift of focus, but rather it is more an implementation of the information we have learned over the past decade. What opportunities for training and professional development has the project provided?Training opportunities -three undergraduate students obtained career jobs or moved on to PhD programs at major universities -four undergraduate students conducted internships at companies or government institutions -eleven undergraduate students are currently conducting focused research to support this project -nine undergraduate students presented research posters at national level plant breeding conferences -two graduate students are coducting PhD dissertation research to support this project How have the results been disseminated to communities of interest?-new cultivars have been released and licensed by the industry - these plants are now being sold to consumers in national retail stores -refereed journal articles are being published to allow the scientific community access to our research results. Our methods for consumer assisted selection have been adopted widely by university and corporate scientists -popular press coverage of superior performance of our new varieties at public field trials across the US has brought significant attention to our research program from the garden community -we have hosted public tours for approximately 10 different and diverse public groups -we continue giving more invited seminars from the US gardening community to discuss our research What do you plan to do during the next reporting period to accomplish the goals?-Continue the coleus breeding program to maintain the pipeline of introductions to the plant industry -continue expansion of our efforts in reeding and selecting new cultivars of downy mildew resistant sweet basil -continue growing our efforts in breeding and selection of new ornamental and edible garden plants.

Impacts
What was accomplished under these goals? This project is now providing significant benefits to players in all aspects of the US horticultural supply chain. As a result of our efforts, plant scientist are gaining a better understanding of the biochemical components of flavor and fragrance in several different plant species. Producers and handlers of horticultural crops are now growing new varieties of plants produced from our work that have good commercial potential, as demonstrated by increased marketing and sales. US consumers are now planting and eating our new plants that look smell and taste better than many others in the marketplace. Main accomplishments: 12 new coleus cultivars were bred and released 1 new basil cultivar was brd and released 10 US plant patent applications were submitted through the Florida Foundation Seed Producers

Publications

  • Type: Journal Articles Status: Awaiting Publication Year Published: 2019 Citation: Jiayi Sun, Cindy Sigler, Guillaume Beaudoin, Jaya Joshi, Jenelle Patterson, Keun Cho, Maria Ralat, Jesse Gregory, David Clark, Zhanao Deng, Thomas Colquhoun, and Andrew Hanson. In press. Parts-prospecting For a High-efficiency Thiamin Thiazole Biosynthesis Pathway. Plant Physiology.
  • Type: Other Status: Submitted Year Published: 2019 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-88-9
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-72-8
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-64-1
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-27-1
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-5-6
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF16-1-20
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Ocimum hybrid UF16-23-2
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF15-20-6
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF15-97-9
  • Type: Other Status: Submitted Year Published: 2018 Citation: US Plant Patent Applied For: Solenostemon scutellarioides UF15-6-28


Progress 10/01/16 to 09/30/17

Outputs
Target Audience:-Human sensory scientists -Plant breeders and biotechnologists -Plant producers -Plant shippers and handlers -Plant marketers -Food scientists -Consumers of plant-based food products Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Training opps: -Two undergraduate students moved on to PhD programs at major US universities. -Five undergraduates remain conducting thesis research for the upcoming year. -All postdoc, grad student and undergraduate students have presented their research results in a formal setting. Some local, some national and others international. How have the results been disseminated to communities of interest?Dissemination of results: -new cultivars have been released and licensed by the industry - these plants are now being sold to consumers in national retail stores. -refereed journal articles are being published, allowing the scientific community to understand more about consumer-assisted selection. University scientists are expanding this new area of scientific interest, and corporate scientists are implementing our ideas into their new commercial products. -Popular press coverage has further spread the word about our research. -we have hosted public tours to approximately 10 different diverse public groups -we have given talks to four separate large groups of Florida Master Gardeners about "consumer assisted selection" of new horticultural crops. What do you plan to do during the next reporting period to accomplish the goals?-Continue the coleus breeding program to continue supplying new cultivars to the plant industry. -Expand our efforts in breeding and selection of new downy mildew resistant basil cultivars. Now that clonally propagated varieties are ready for the industry, our efforts will go toward development of inbred seeds lines. -Grow our efforts in breeding and selection of new ornamental and edible plant crops. We are currently screening several genera and species for potential breeding targets.

Impacts
What was accomplished under these goals? This project has the potential to provide significant benefits to virtually every player in the supply chain for US horticultural crops. Plant scientists will gain a better understanding of the biochemical components of flavor, fragrance and taste in several different plants. Producers and handlers of horticultural crops will have new varieties of plants with better commercial potential. Marketing and business people will gain a better understanding of the drivers of human liking of plants and plant products.Ultimately US consumers will get new fruits, vegetables and plants that look, smell and taste better. Accomplishments: -Three (3) US plant patents were issued for coleus cultivars we bred and released from this project. -One refereed manuscript was produced, two were accepted pending revisions and two others were submitted -The new basil breeding program was expanded and had now produced superior heat tolerant plants with confirmed downy mildew resistance. The first new cultivar from this program is being released.

Publications

  • Type: Other Status: Published Year Published: 2017 Citation: US Plant Patent PP 28,591: A coleus plant named UF 13-26-7
  • Type: Other Status: Published Year Published: 2017 Citation: US Plant Patent PP 28,566: A coleus plant named UF 13-42-4
  • Type: Other Status: Published Year Published: 2017 Citation: US Plant Patent PP 28,517: A coleus plant named 'UF 13-48-27'
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Mennella, J., Colquhoun, T. A., Bobowski, N.K., Olmstead, J.W., Bartoshuk, L., and Clark, D.G. 2017. Farm to Sensory Lab: Taste of Blueberry Fruit by Children and Adults. Journal of Food Science 82:1713-1719.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:-Human sensory scientists -Plant breeders and biotechnologists -Plant producers -Plant shippers and handlers -Plant marketers -Food scientists -Consumers of plant-based food products Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?-One MS student graduated this year and successfully defended her dissertation. -One postdoc (and former graduate student) was employeed in a high-level technical job in the small fruit industry. -Three undergraduate students moved on to PhD programs at major US universities. -Six undergraduates remain conducting honors thesis research for the upcoming year. -All postdoc, grad student and undergraduate students have presented their research results in a formal setting. Some local, some national and others international. -A UF/IFAS student research scholar program was implemented through the coleus breeding program. How have the results been disseminated to communities of interest?-New cultivars have been released, patentedand licensed by the industry - these plants are now being sold to consumers in national retail stores. -Refereed journal articles have been published, allowing the scientific community to understand more about consumer-assisted selection. University scientists are expanding this new area of scientific interest, and corporate scientists are implementing our ideas into their new commercial products. -Popular press coverage has further spread the word about our research. In the past year, we have been covered in stories from the London Telegraph, Los Angeles Times, Wall Street Journal, and several trade publications. -We have hosted public tours to approximately 20 different diverse public groups ranging from K-12 students to master gardeners to legislators. -I have given talks to 3 separate large groups of Florida Master Gardeners about "consumer assisted selection" of new horticultural crops. What do you plan to do during the next reporting period to accomplish the goals?-Maintain the coleus breeding program to continue supplying new cultivars to the plant industry. -Expand our efforts in breeding and selection of new downy mildew resistant basil cultivars. -Maintain our efforts in breeding and selection of new ornamental blueberry cultivars. -Maintain our efforts in breeding and selection of new garden hot pepper cultivars. -Continue the implementation of the UF/IFAS Coleus Research Scholars Program.

Impacts
What was accomplished under these goals? -Five (5) US plant patents were issued for coleus cultivars we bred and released from this project. -Three (3) refereed manuscripts were produced from results generated by this project. -The UF/IFAS Plant Innovation Center has continued to grow and expand, with new projects being funded by extramural institutions and partners. -The new basil breeding program was expanded and had now produced superior heat tolerant plants with confirmed downy mildew resistance. -Selections from the ornamental blueberry program are now being tested in commercial trials. -A new garden hot pepper effort was established to produce new cultivars for home garden use.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Dewar, P. E., Keene, S. A., Kalk, T. N., Clark, D. G., & Colquhoun, T. A. (2016). Identifying the Drivers of a Foliage Plant Purchasing Decision via Contemporary Psychophysics. J Hortic, 3(177), 2376-0354.
  • Type: Journal Articles Status: Accepted Year Published: 2016 Citation: Johnson, T. S., Schwieterman, M. L., Kim, J. Y., Cho, K. H., Clark, D. G., & Colquhoun, T. A. (2016). Lilium floral fragrance: A biochemical and genetic resource for aroma and flavor. Phytochemistry, 122, 103-112.
  • Type: Journal Articles Status: Submitted Year Published: 2017 Citation: Julie A. Mennella, Thomas A. Colquhoun, Nuala K. Bobowski, James W. Olmstead, Linda Bartoshuk, and Dave Clark (submitted) Farm to sensory lab: Taste of blueberry fruit by children and adults. Journal of Food Science.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:Potential Target Audiences: -Human sensory scientists -Plant breeders and biotechnologists -Plant producers -Plant shippers and handlers -Plant marketers -Food scientists -Consumers of plant-based food products Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?-One PhD student graduated this year and successfully defended her dissertation. She was immediately employeed as a plant breederin the small fruitindustry. -One postdoc (and former graduate student) was employeed in a high-level technical job in the small fruit industry. -Two undergraduate students moved on to PhD programs at major US universities. -Four undergraduates remain conducting honors thesis research for the upcoming year. -All postdoc, grad student and undergraduate students have presented their research results in a formal setting. Some local, some national and others international. -A UF/IFAS student research scholar program was conceived with implementation ongoing. How have the results been disseminated to communities of interest?-new cultivars have been released and licensed by the industry - these plants are now being sold to consumers in national retail stores. -refereed journal articles have been published, allowing the scientific community to understand more about consumer-assisted selection. University scientists are expanding this new area of scientific interest, and corporate scientists are implementing our ideas into their new commercial products. -Popular press coverage has further spread the word about our research. In the past year, we have been covered in stories from the New York Times, Los Angeles Times, Wall Street Journal, and National Geographic magazine. -we have hosted public tours to approximately 25 different diverse public groups ranging from K-12 students to master gardeners to legislators. What do you plan to do during the next reporting period to accomplish the goals?-Maintain the coleus breeding program to continue supplying new cultivars to the plant industry. -Expand our efforts in breeding and selection of new downy mildew resistant basil cultivars. -Expand our efforts in breeding and selection of new ornamental blueberry cultivars. -Establish and implement the UF/IFAS Plant Innovation Center Research Scholars Program.

Impacts
What was accomplished under these goals? -Six (6) US plant patents were issued for coleus cultivars we bred and released from this project. -Three (3) refereed manuscripts were produced from results generated by this project. -The UF/IFAS Plant Innovation Center wasformalized around our concept of consumer-assisted selection of plants that consumers like best. -A new basil breeding program was established to provide heat tolerant plants with downy mildew resistance. -A new ornamental blueberry program was established to produce new cultivars for home garden use.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Olmstead, M.A., Gilbert, J.L., Colquhoun, T.A., Clark, D.G., Kluson, R., and Moskowitz, H.R. 2015. In pursuit of the perfect peach: consumer-assisted selection of peach fruit traits. HortScience. 50:1202-1212.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Gilbert, J.L., Guthart, M.J., Gezan, S.A., de Carvalho, M.P., Schwieterman, M.L., Colquhoun, T.A., Bartoshuk, L.M., Folta, K.M., Sims, C.A., Clark, D.G., and Olmstead, J.W. 2015. Breeding better blueberries: biochemistry of the flavor experience and genetic and environmental variation. PLoS ONE 10(9): e0138494.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Johnson, T.S., Schwieterman, M.L., Kim, J.Y., Cho, K.H., Clark, D.G., and Colquhoun, T.A. 2015. Lilium floral fragrance: a biochemical and genetic resource for aroma and flavor. Phytochem. doi:10.1016/j.phytochem.2015.11.010.


Progress 07/02/14 to 09/30/14

Outputs
Target Audience: The targeted audiences for this research project cover a broad range of fields related to all points in the supply chain forflowers, fruits and vegetables. Through the process of consumer-assisted selection, we first address consumers of specific plant crops/products to determine which traits they like best. We then communicate this information to plant scientists involved in plant breeding and biotechnology as a means to instruct them on the most impactful breeding targets. We also communicate with producers, shippers and supplies of these products, and provide information to consumer marketing experts. ultimately this information goes out to consumers and the public through various media formats. Changes/Problems: We see no major changes in this project in the year ahead. What opportunities for training and professional development has the project provided? This project has been the focus of research for one postdoctoral associate. One graduate student and two undergraduate students contributed to the most recent refereeed manuscript, and two other undergraduate students are now working on this project. How have the results been disseminated to communities of interest? Results from this project have been disseminated through the publication of a refereed manuscript. A press release of this work was taken forward from our institution, and a small number of media outlets ran subsequent stories on the topic. We have also recently created a small scientific writing group among the students working in our lab, and they will be working this next year to distill the results of this and other current work into forms more readily available for popular press and consumers. What do you plan to do during the next reporting period to accomplish the goals? The main goal of our project is to conduct research focused on bridging the gap between basic and applied plant science through the genetic improvement of various horticultural crops. We hope to expand our repertoire of high-value Florida crops and products being developed through the process of Consumer-assisted Selection beyond strawberry. We plan to go forward with similar basic and applied research on other important Florida crops such as blueberry, tomato, basil and ornamental plants such as coleus.

Impacts
What was accomplished under these goals? The flavor of fresh strawberries is one of the most pleasurable of all fruits; comprised of simple sugars, a few organic acids, and a complex mixture of volatile compounds. As a result of our work individual sugars and acids were quantitated in fruit, as well as 81 volatile compounds potentially contributing strawberry flavor. Simultaneous to chemical inventory, 100 consumers rated each variety for its flavor intensity, sweetness, and overall liking. To expand the range and diversity of flavors and chemical constituents 35 genetic backgrounds were grown over two seasons. Analysis of this multidimensional data set revealed seasonal effects, gross variation across varieties, and fruit attributes influencing liking and sensory perception of strawberry fruit. Seasonal progression exerts a consumer perceivable, negative influence on sugar content in subtropical Florida, leading to decreased volatile content. The sweeter and more flavorful biochemical profile of early harvests is grounds for producers to demand premium prices. Interestingly, some of the most abundant volatiles do not contribute to consumer liking, whereas other less abundant volatiles significantly increase flavor intensity. In strawberry, we discovered a subset of specific volatiles that make contributions to perceived sweetness independent of sugar concentration in the fruit.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Schwieterman ML, Colquhoun TA, Jaworski EA, Bartoshuk LM, Gilbert JL, Tieman DM, Odabasi AZ, Moskowitz HR, Folta KM, Klee HJ, Sims CA, Whitaker VM, and Clark DG (2014) Strawberry Flavor: Diverse Chemical Compositions, a Seasonal Influence, and Effects on Sensory Perception. PLoS ONE 9(2): e88446. doi:10.1371/journal.pone.0088446