Source: NORTHWEST INDIAN COLLEGE submitted to NRP
NUTRITIONAL VALUE OF FINFISH AND SHELLFISH ESSENTIAL TO LUMMI DIETS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
1013597
Grant No.
2017-38424-27084
Cumulative Award Amt.
$190,182.00
Proposal No.
2017-03928
Multistate No.
(N/A)
Project Start Date
Sep 1, 2017
Project End Date
Aug 31, 2021
Grant Year
2017
Program Code
[ZY]- Tribal Colleges Research Grants Program
Recipient Organization
NORTHWEST INDIAN COLLEGE
2522 KWINA ROAD
BELLINGHAM,WA 98226-9278
Performing Department
Salish Sea Research Center
Non Technical Summary
Seafood is rich in omega-3 fatty acids such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and docosapentaenoic acid (DPA). These fatty acids are important for normal metabolism and may be linked to lower incidences of cardiovascular disease. The Lummi People have harvested seafood for commercial, ceremonial, and subsistence purposes since time immemorial. Lummis eat a greater amount and variety of seafood than the average Washington resident. Although seafood is a critical part of Lummi tribal member diets, there is little information on the nutritional value of many species of finfish and shellfish they normally consume, especially regarding fatty acids. For instance, there is no information on the fatty acid content of horse clams (Tresus spp.) or butter clams (Saxidomus gigantea), which are widely eaten by Lummis. Another case is Dungeness crab, a Lummi diet staple for which there are no recent fatty acid studies in the Salish Sea. Many finfish typically consumed by tribal members have also not been well characterized. For example, there is no information on the fatty acid composition of longfin smelt (Spirinchus thaleichthys) from the Nooksack River. Our main goal is to study the nutritional value of seafood species that are vital to Lummi tribal member diets. We will measure the concentration of different fatty acids in seven species of shellfish and one species of finfish, gaining information on which foods offer the best nutrition. We also aim to increase our capacity to do fatty acid research and train the next generation of Native researchers.
Animal Health Component
0%
Research Effort Categories
Basic
100%
Applied
0%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
70108111010100%
Knowledge Area
701 - Nutrient Composition of Food;

Subject Of Investigation
0811 - Shellfish;

Field Of Science
1010 - Nutrition and metabolism;
Goals / Objectives
Goal 1: Measure the fatty acid profile of Nooksack longfin smeltObjective 1: Measure the concentration of different fatty acids in longfin smelt to determine its nutritional valueObjective 2: Compare the fatty acid composition and concentration of male longfin smelt to that of female longfin smeltGoal 2: Measure the fatty acid profiles of shellfish on the Lummi ReservationObjective 1: Measure the concentration of different fatty acids in butter clams, horse clams, littleneck clams, Manila clams, Pacific oysters, red rock crab, and Dungeness crab to determine their nutritional valueGoal 3: Increase the capacity for Land Grant research at NWICObjective 1: Increase capacity to do fatty acid analyses via the purchase of a freeze dryer and nitrogen evaporatorObjective 2: Train researchers to operate a freeze dryerObjective 3: Continue to train researchers and student interns to operate the Shimadzu GC-2010 GC-MSObjective 4: Train researchers and student interns on data collection, sample analysis, and results interpretation of fatty acids in animal tissueGoal 4: Increase indigenous scholars in STEM through placed based Land Grant ResearchObjective 1: Support NWIC students in paid research internshipsObjective 2: Increase awareness of NWIC Land Grant research with Native youth in the area
Project Methods
Overall project designLongfin smelt swim up the Nooksack River around November of every year to spawn. Twenty individuals were collected by Lummi Natural Resources (LNR) in November 2016 and frozen. Both females and males will be sampled to ensure that sex is not a confounding factor, as females may have greater lipid stores than males. Twenty specimens of each shellfish species (butter clams, horse clams, littleneck clams, Manila clams, Pacific oysters, red rock crab, and Dungeness crabs) will be collected from tide flats and marine waters on the Lummi Reservation. For clams and oysters, tissue from the adductor muscle will be extracted, whereas for crabs, leg meat will be collected. Prior to lipid extraction, tissues will be freeze dried at <-50oC for 48h.Fatty acid analysesFatty acid extractions will be based on a modified Bligh & Dyer (1959) technique, using a one-step saponification transesterification approach following Lewis et al. (2000) as modified by Thurber et al. (2013). Preliminary tests will determine the optimal amount of freeze-dried tissue for fatty acid extraction and analysis. The exact mass used is measured to standardize the fatty acid concentrations in all samples. We will also use an internal fatty acid standard of nonadecanoic acid (C19:0) to allow quantitative calculation of mg of specific fatty acids (both beneficial and otherwise) per g of tissue.All samples will be analyzed in NWIC's Shimadzu GCMS-2010 gas chromatograph-mass spectrometer. Fatty acid methyl esters (FAMEs) will be separated with a DB-5 capillary column with helium as the carrier gas. FAMEs are identified via mass spectrometry by comparing their retention times with a Supelco 37 Component FAME Mix (Sigma-Aldrich) and verified with the NIST Mass Spectral Library. FAME concentrations are calculated by converting the area of the fatty acid peaks in the mass chromatogram to weight using the known nonadecanoic acid internal standard as a reference. While mass spectrometers provide information about the identity of the fatty acids being analyzed they are not, by themselves, quantitative as MS response is a function of chain length, double bond, and branching pattern. However, they are internally reproducible and thus through comparison to a GC- Flame Ionization detector that has been calibrated, quantitative results are possible. To obtain this, a subset (as needed) of the samples analyzed within the NWIC GC-MS will also be run in the lab of Oregon State University's co-PI Thurber's GC-FID to allow calibration and quantification. This will likely be needed on a subset of the samples and will provide the ability for quantitative data to be generated by the NWIC GC-MS. Students from NWIC will travel to OSU to help with these analyses and gain experience on a second GC system (a Thermo 1310 GC-FID). A subsample of freeze-dried tissue will be sent to the Oregon State University's Stable Isotope Facility for 13C analysis. The carbon to dry biomass measurement is used to standardize fatty acid concentrations to carbon content.Data analysis Data will be analyzed during all stages of this research project and during a write up session involving all partners in year two of the project. Overall changes in the fatty acid composition among different sources will be visualized using multi-dimensional scaling (MDS) and ANOSIM analyses will allow test whether the overall fatty acid signatures differ among food sources. Those fatty acids that separate the food sources will be identified using the SIMPER test. We will also focus on the abundance of those fatty acids that are most beneficial to consumers (i.e. people) by testing the difference in EPA and DHA among the different food sources using a one-way ANOVA. Statistical analysis will be performed in the free software R, using the statistical packages VEGAN and those tools natively in R. We have chosen this analysis pathway as it is a powerful tool that both exposes the students (who will be integral to the analysis) a common programming tool and its ubiquity in biological science research. Land Grant Partner Thurber will help teach and troubleshoot R to the students during the analysis phase of this project.

Progress 09/01/17 to 08/31/21

Outputs
Target Audience:The target audience is fpr tribal undergraduate students at Northwest Indian College, Pacific Northwest Tribal sustenance and recreational seafood harvesters, Lummi Natural Resources, and Native youth groups. This project has directly employed two student interns and twelve undergrad students taking the year-long inorganic chemistry series at Northwest Indian College. We routinely converse with commercial, recreational, and subsistence Native fishers while collecting samples. This work has already been presented at local conferences to the scientific community and public. Changes/Problems:Due to COVID 19, we have not been able to complete our analyses. We have a continuing relationship with our research I partner at OSU, and will continue to collaborate to analyze the fatty acid content of Native foods and provide opportunities to engage in further food research beyond the life of this project. What opportunities for training and professional development has the project provided?Due to COVID 19 closures, there have been no opportunities for professional development. We anticipate that more opportunities in this area will arise as COVID 19 restrictions are eased. How have the results been disseminated to communities of interest?We continue to partner with Lummi Natural Resources to disseminate project updates to our communities of interest, which includes the student's home communities. Students who received Indigenous food samples from community elders have continuedto engage their traditional teachers to acquire knowledge related to the culture and collection of Native foodresources. Students will report back to their tribal communities when results are completed after analysis at OSU. To date, the project results have been disseminated at regional conferences, and NIFA support has been aknowledged in those presentations. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Goal 1: Measure the fatty acid profile of Nooksack longfin smelt Objective 1: Measure the concentration of different fatty acids in longfin smelt to determine its nutritional value Objective 2: Compare the fatty acid composition and concentration of male longfin smelt to that of female longfin smelt Students at Northwest Indian College extracted an extensive set of fatty acid samples from male and female longfin smelt. These samples were sent to Oregon State university for analysis. Due to COVID 19 and technical issues at OSU, the samples have not been fully analyzed. Students from Northwest Indian College gave a presentation on their participation in the fatty acid project at the Society for the Advancement of Chicanos and Native Students (SACNAS). Goal 2: Measure the fatty acid profiles of shellfish on the Lummi Reservation Objective 1: Measure the concentration of different fatty acids in butter clams, horse clams, littleneck clams, Manila clams, Pacific oysters, red rock crab, and Dungeness crab to determine their nutritional value. Students from Northwest Indian College collected an extensive range of samples from native shellfish traditionally used for subsistence, including littleneck, butter, manila, razor, varnish, horse, and geoduck clams. Beyond this, the students collected fatty acidsamples from an array of other traditional foods, including chiton, halibut andherring eggs. Additionally, the studentsweregifted with samples of elk, deer, and harbor seal collected by traditional gatherers from their home villages. These samples are being stored at OSU, and will be analyzed when the impacts of the pandemic permit labs to return to full capacity. Goal 3: Increase the capacity for Land Grant research at NWIC Objective 1: Increase capacity to do fatty acid analyses via the purchase of a freeze dryer and nitrogen evaporator. A freeze dryer was purchased and used for the fatty acid project. The freeze dryer will continue to be used at Northwest Indian College (NWIC) to support inquiry into the nutritional value of Native foods, electron microscopy, and a diverse set of other research topics. Objective 2: Train researchers to operate a freeze dryer. Researchers at NWIC are well-trained in the use of the freeze dryer, and have taught students to use this device as well. Objective 3: Continue to train researchers and student interns to operate the Shimadzu GC-2010 GC-MS. he GC-MS at NWIC is unfortunately inoperable. Due to the COVID panademic, it is not possible to secure technical support to repairthe instrument, and furthermore, it is dubious that the GC-MS would become operable even with expert support. Objective 4: Train researchers and student interns on data collection, sample analysis, and results interpretation of fatty acids in animal tissue Researchers and students at NWIC are now proficient in all phase of fatty acid sample collection and preparation.? We await the data from our partners at OSU to analyze the results and report on the fatty acid content of tradional Native foods. Goal 4: Increase indigenous scholars in STEM through placed-based Land Grant Research Objective 1: Support NWIC students in paid research internships. Due to COVID 19, NWIC was not allowed to have interns on campus during this reporting period. Objective 2: Increase awareness of NWIC Land Grant research with Native youth in the area. Through the fatty acid project, Northwest Indian College became more aware of the possibilities of land grant research to support tribal sovereignty and self-determination. Their friends and families in their home communities became enthusiastic about this research as well, sending in samples of traditonal foods for analysis

Publications


    Progress 09/01/19 to 08/31/20

    Outputs
    Target Audience:The target audience is for tribal undergraduate students at Northwest Indian College, Pacific Northwest Tribal sustenance and recreational seafood harvesters, Lummi Natural Resources, and Native youth groups. This project has directly employed two student interns and twelve undergrad students taking the inorganic chemistry year-long class at Northwest Indian College. We routinely converse with commercial, recreational, and subsistence Native fishers to while collecting samples. This work has also already been presented at local conferences to the scientific community and public. Changes/Problems:Due to COVID-19 restrictions and unanticipated issues related to travel and closures we were unable to complete the objective of student learning instrumentation at OSU, as well as hiring and completing summer student internships for summer 2020. The PI/Co-Is are working together to figure out a remote internship for two students who already participated in this project and are analyzing the data for their senior thesis capstones, as well as setting up a (safe) rotating schedule for students to learn lab techniques related to this project in the coming fall. It is not clear yet if students will be allowed in the lab fall quarter due to COVID-19. We are requesting a second 12-month NCE to complete this grant because of the COVID-19 shutdown and will soon have both a plan to complete physical objectives, and a contingency plan if we can only participate in remote learning. What opportunities for training and professional development has the project provided?This project has provided numerous opportunities for professional development. 28 student researchers at NWIC have learned a multitude of fatty acid instruments, including freeze drying, wet chemistry, and GC/MS instrumentation. Students have also learned field and laboratory methods and analyses related to this project, and have spoken with elders and knowledge holders to guide them in choices for what Indigenous foods to sample for fatty acids. All 16 student researchers who were enrolled in the Chemistry class series for this reporting period presented their research at the Chemistry symposium at Northwest Indian College. Two student researchers presented their research at a local and/or regional conferences. Acknowledgement for this grant was included in the presentations. Students anticipated travel to OSU, our land grant partner in in spring 2020, but due to COVID-19 restrictions both labs (NWIC and OSU) were closed and travel was not allowed. How have the results been disseminated to communities of interest?We continue to partner with Lummi Natural Resources to disseminate project updates to our communities of interest, which includes the student's in the chemistry classes' individual communities. Students who received Indigenous food samples from elders have continued to engage them in the processing of the samples, the experience, and the results received from OSU. Students will report back to their tribal communities when results are completed after analysis at OSU. To date the project results have been disseminated at regional conferences, and NIFA support has been acknowledged in those presentations. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period samples will be analyzed for fatty acids at OSU (land-grant partner). We hope to allow students and researchers to travel to our land-grant partner at OSU for training and analysis, which we were unable to complete this year due to COVID-19. The PI/Co-Is are working together to provide a contingency plan in case of continued COVID-19 closures. The lab at NWIC was closed in March 2020 due to COVID-19, but has recently started to allow researchers back in a shortened, alternating schedule. Most of the samples had been processed in anticipation of final analysis at OSU, so the last subset of samples will be processed at NWIC. We anticipate allowance of travel in 2021, and hope to have students participate in the analysis portion of the project at OSU, but in case that is not available, we will ship samples to OSU for analysis without the students. We are working together to set up a video/zoom link to learn/watch the analysis process if physical presence at OSU is not available.

    Impacts
    What was accomplished under these goals? Goal 1: Measure the fatty acid profile of Nooksack longfin smelt Objective 1: Measure the concentration of different fatty acids in longfin smelt to determine its nutritional value Objective 2: Compare the fatty acid composition and concentration of male longfin smelt to that of female longfin smelt This project was able to utilize the 2019 Longfin smelt run from the Nooksack River, as well as analyze historical samples from 2016 and 2017. The first set of fatty acid data has been analyzed and completed at OSU (land grant partner) on their GC-MS. The students in the Northwest Indian College in the Inorganic Chemistry series class used this data for a class project and presented it at a chemistry symposium. PI Rombold and Co-I Thurber are working with the students to interpret this data. Goal 2: Measure the fatty acid profiles of shellfish on the Lummi Reservation Objective 1: Measure the concentration of different fatty acids in butter clams, horse clams, littleneck clams, Manila clams, Pacific oysters, red rock crab, and Dungeness crab to determine their nutritional value Samples from local butter, horse, Manila, varnish, and little neck clams were collected and processed for analysis in summer 2019. Dungeness and red rock crab have also been processed. Other Indigenous foods such as salmon, and forage fish have also been collected and processed. Students during the summer 2019 season as well as fall 2020 supplemented these samples with Indigenous foods from Navajo Nation and Native Alaskan tribes, and learned how to process them in the lab. Each student reached out to their elders or communities to ask for Indigenous foods to be analyzed and reported the experience back to their communities. Goal 3: Increase the capacity for Land Grant research at NWIC Objective 1: Increase capacity to do fatty acid analyses via the purchase of a freeze dryer and nitrogen evaporator Objective 2: Train researchers to operate a freeze dryer Objective 3: Continue to train researchers and student interns to operate the Shimadzu GC-2010 GC-MS These instruments were purchased in 2018/2019, and the freeze dryer and evaporator continues to be a multi-researcher and student class use instrument. This project was originally conceived to have targeted science students participate, but has evolved to be written into the chemistry lab curriculum at Northwest Indian College. For a second year, students from the year-long inorganic chemistry class learned how to process and analyze fatty acids, including the use of the freeze dryer and nitrogen evaporator. Students from the chemistry class were expected to travel to Oregon State University in the spring of 2020, to complete the analysis on the GC-MS but were unable to do so because of COVID-19 shelter in place orders. A subset of samples were sent in fall (September/October) 2019 to be process at OSU, and the students in the inorganic chemistry classes used that data to present for the chemistry class series and supplement the laboratory work. One graduate student mentor that worked with NWIC students in the summer 2019 working with Co-I Thurber at OSU to process the student's samples at OSU in Fall 2019 and Winter 2020. This graduate mentor is continuing to help the students process the data and work with them on learning the GC-2010 GC-MS. Goal 4: Increase indigenous scholars in STEM through placed based Land Grant Research Objective 1: Support NWIC students in paid research internships Three undergraduate Northwest Indian College students were paid off of this grant in the last reporting period, completing intern projects related to fatty acid extraction of Indigenous foods (for a total of 6 paid internships). An additional 16 students this reporting period (28 total) have been exposed to this project through the chemistry lab series that taught the students how to extract fatty acids from a variety of Indigenous foods. Results from this data is being used in both the chemistry class, as will be used as a statistical data set for biostatistics class in the next reporting period. Three students have presented this work at conferences. The entire chemistry class (16 students) presented their work to their communities. Another student presented this research as an invited speaker. One student used this research to complete their undergraduate senior thesis capstone. Objective 2: Increase awareness of NWIC Land Grant research with Native youth in the area Elements of this project, such as clamming, finfish fishing, field collection methods, and shellfishery and finfish management were incorporated to four weekly lessons at for Lummi Nation School 3rd and 5th graders . An instructional primer for the third graders with these lessons has also been used at Quileute Tribal School during their summer science camps. Due to COVID-19 physical closures, we are working to create an online module of this work for K-12 students. This will provide a wider distribution audience.

    Publications

    • Type: Theses/Dissertations Status: Published Year Published: 2020 Citation: Milne, Mikale. (2020). Coast Salish Intertidal Foods. Undergraduate senior thesis capstone. Northwest Indian College, Bellingham, WA.
    • Type: Conference Papers and Presentations Status: Published Year Published: 2019 Citation: Brewer, M. and J. Rombold. 2019. Omega -3 Fatty Acid Content of Traditional Foods from the Salish Sea. SACNAS. Honolulu, HA, October29-November2 2019.
    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: Cooper, K. and J. Rombold. Omega-3 Fatty Acids in Native American Traditional Food. SACNAS. Honolulu, HA, October29-November2 2019.
    • Type: Conference Papers and Presentations Status: Published Year Published: 2020 Citation: Rombold, J. et al (2020). An assessment of fatty acids of Indigenous foods. Chemistry symposium, Northwest Indian College.


    Progress 09/01/18 to 08/31/19

    Outputs
    Target Audience:The target audience is for tribal undergraduate students at Northwest Indian College, Pacific Northwest Tribal sustenance and recreational seafood harvesters, Lummi Natural Resources, and Native youth groups. This project has directly employed two student interns and twelve undergrad students taking the inorganic chemistry year-long class at Northwest Indian College. We routinely converse with commercial, recreational, and subsistence Native fishers to while collecting samples. This work has also already been presented at local conferences to the scientific community and public. Changes/Problems:Due to instrument down time at OSU and scheduling to allow our students to continue participation in all aspects of this project we have not completed analyses of fatty acid samples and have asked and been approved for a 12-month NCE to complete this work. We anticipate finishing this project by August 2020. What opportunities for training and professional development has the project provided?This project has provided numerous opportunities for professional development. 14 student researchers at NWIC have learned a multitude of fatty acid instruments, including freeze drying, wet chemistry, and GC/MS instrumentation. Students have also learned field and laboratory methods and analyses related to this project, and have spoken with elders and knowledge holders to guide them in choices for what Indigenous foods to sample for fatty acids. All twelve student researchers who were enrolled in the Chemistry class series presented their research at the Chemistry symposium at Northwest Indian College. Two student researchers anticipate presenting their research at a local and/or regional conference in the next reporting period. Acknowledgement for this grant will be included in the presentations. Students will also travel to OSU, our land grant partner in the upcoming reporting period in order to participate in sample analysis. ? How have the results been disseminated to communities of interest?We continue to partner with Lummi Natural Resources to disseminate project updates to our communities of interest. Students who received Indigenous food samples from elders have continued to engage them in the processing of the samples. Students will report back to their tribal communities when results are completed after analysis at OSU. To date the project results have been disseminated at regional conferences, and NIFA support has been acknowledged in those presentations. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period samples will be analyzed for fatty acids, and students and researchers will travel to our land-grant partner at OSU for training and analysis. There will be a PD/Co-D meeting during the next reporting period to follow the progress of this project and also to analyze and write-up the data. This data will also be presented by students at a regional conference.

    Impacts
    What was accomplished under these goals? Elements of this project, such as clamming, finfish fishing, field collection methods, and shellfishery and finfish management were incorporated to four weekly lessons at for Lummi Nation School 3rd and 5th graders . An instructional primer for the third graders with these lessons has also been used at Quileute Tribal School during their summer science camps.

    Publications

    • Type: Conference Papers and Presentations Status: Accepted Year Published: 2019 Citation: J. Rombold, et. al. (2019). An assessment of fatty acids of Indigenous foods. Chemistry symposium, Northwest Indian College.


    Progress 09/01/17 to 08/31/18

    Outputs
    Target Audience:Our target audience is Lummi Natural Resources management, Lummi Nation commercial shell fishers and fishermen, and Native youth groups. During this project period, the project directly employed one NWIC undergrad and engaged 20+ students through classroom activities. This project has been discussed with Lummi Natural Resources management. Changes/Problems:The PD for this grant has been switched, as the previous PD, Andres Quesada, is no longer employed at NWIC. Dr. John Rombold, Faculty at Northwest Indian College and Chemistry Professor for the Bachelor of Science in Native Environmental Science program is the PD of this grant. Dr. Melissa Peacock and Dr. Andrew Thurber, Co-Is on this grant continued the project during this PD change. What opportunities for training and professional development has the project provided?One researcher and one student on this project has traveled to our land-grant partner (OSU) to be trained on fatty acid extraction and analysis, as well as learning to use the two new instruments, the freeze dryer and N2 dehydrator. One researcher participated in a NIFA review panel. How have the results been disseminated to communities of interest?Preliminary results have been discussed with Lummi Natural Resources managers. Progress updates have been disseminated to communities of interest through development of native curricula, through targeted briefings, and through conference presentations. What do you plan to do during the next reporting period to accomplish the goals?During the next reporting period the last field samples will be collected, analyzed for fatty acids, and students and researchers will travel to our land-grant partner at OSU for training and analysis. There will be a PD/Co-D meeting during the next reporting period to follow the progress of this project and also to analyze and write-up the data. This data will also be presented at SACNAS by students next year.

    Impacts
    What was accomplished under these goals? Goal 1: Measure the fatty acid profile of Nooksack longfin smelt ?Objective 1: Measure the concentration of different fatty acids in longfin smelt to determine its nutritional value Objective 2: Compare the fatty acid composition and concentration of male longfin smelt to that of female longfin smelt The longfin smelt run (locally known as the Hooligan) happened from November 10-20th, 2018, adding a second year of longfin smelt data for fatty acid analysis. Samples are being processed for fatty acid extraction. Researchers visited Oregon State University (our Land-Grant partner) to learn the analysis and processing techniques for fatty analysis extraction, and will use this methodology to complete data analysis on longfin smelt collected for both 2017 and 2018. Goal 2: Measure the fatty acid profiles of shellfish on the Lummi Reservation Objective 1: Measure the concentration of different fatty acids in butter clams, horse clams, littleneck clams, Manila clams, Pacific oysters, red rock crab, and Dungeness crab to determine their nutritional value All clam field samples have been collected, and are ready for fatty acid extraction and analysis. Crab and oyster samples will be collected in January and frozen for analysis. Goal 3: Increase the capacity for Land Grant research at NWIC Objective 1: Increase capacity to do fatty acid analyses via the purchase of a freeze dryer and nitrogen evaporator Objective 2: Train researchers to operate a freeze dryer Both a freeze dryer and nitrogen evaporator have been purchased and students and researchers on this project have been trained on their use. Objective 3: Continue to train researchers and student interns to operate the Shimadzu GC-2010 GC-MS One researcher has been trained extensively on this instrumentation, and we expect to have other researchers and students trained throughout the project. Objective 4: Train researchers and student interns on data collection, sample analysis, and results interpretation of fatty acids in animal tissue Students were involved (and will be) in all aspects of the field collection, and the Chemistry 122 undergraduate class will be trained on how to sample, analyze and interpret results as part of a year-long chemistry place-based laboratory methodology training. Goal 4: Increase indigenous scholars in STEM through placed based Land Grant Research Objective 1: Support NWIC students in paid research internships One student has been supported on this project so far, and we expect more students to be supported on this project through travel to our Land-Grant partner, and a summer research internship in 2019. Objective 2: Increase awareness of NWIC Land Grant research with Native youth in the area Elements of this project, such as clamming, finfish fishing, field collection methods, and shellfishery and finfish management were incorporated to four weekly lessons at for Lummi Nation School 3rd and 5th graders . An instructional primer for the third graders with these lessons has also been used at Quileute Tribal School during their summer science camps.

    Publications

    • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Peacock, M. and T. Yazzie. 2018. Engaging Northwest Indian College Students and the Tribal Community in Marine Outreach. Ocean Sciences Meeting, Portland, OR.
    • Type: Conference Papers and Presentations Status: Published Year Published: 2018 Citation: Thurber, A.; M.A. Hatch; A. Quesada. 2018. Creating Impacts Through Meaningful Undergraduate Field-Lab Experiential Learning; Lessons Learned Through Collaborations with a Tribal College. Ocean Sciences Meeting, Portland, OR. (Poster)